Detectors: Evaporative Light Scattering
نویسنده
چکیده
Rcation of short-lived free radicals by an electron spin resonance trapping technique. Journal of the American Chemical Society 90: 5909}5910. Kroll C, Osterloh K, MaK der K and Borchert H-H (1998) On-line coupled HPLC-ESR: Application for separation and characterization of organic radicals. Archiv der Pharmazie } Pharmaceutical and Medicinal Chemistry 331 (2): 40. Osterloh K, Kroll C, MaK der K, Borchert H-H, Sammler B and Kirmse C (1998) HPLC und Elektronenspinresonanz-Spektroskopie on-line gekoppelt [On-line Coupling of HPLC and Electron Spin Resonance Spectroscopy] (German). GIT Labor-Fachzeitschrift 42: 24}25. Poole CP (1983) Electron Spin Resonance, 2nd edn. New York: John Wiley. Poole CP and Farach HA (1994) Handbook of Electron Spin Resonance: Data Sources, Computer Technology, Relaxation, and Endor. Berlin: Springer. Sugata R, Iwahashi H, Ishii T and Kido R (1989) Separation of polyunsaturated fatty acid radicals by highperformance liquid chromatography with electron spin resonance and ultraviolet detection. Journal of Chromatography 487: 9}16. Suzuki S, Nakazawa H, Fujita M, Ono S, Suzuki M, Takitani S, Sonoda M and Sakagishi Y (1992) Flow analysis of UV-irradiated chemicals by chemiluminescence and electron spin resonance spectroscopy. Analytica Chimica Acta 261: 39}43.
منابع مشابه
Detectors for High-performance Liquid Chromatography of Lipids with Special Reference to Evaporative Light-scattering Detectio
A. Introduction B. Optical and Spectrophotometric Detectors 1. Differential refractometry 2. Ultraviolet spectrophotometry 3. Fluorescence detection 4. Infrared spectrophotometric detectors 5. Spectrophotometric detection with post-column chemical reaction C. Some Miscellaneous Detection Systems 1. The mass spectrometer as an HPLC detector 2. Radioactivity detectors 3. Density, electrochemical ...
متن کاملPerformance Characteristics of the Agilent 1290 Infinity Evaporative Light Scattering Detector
The Agilent 1290 Infinity Evaporative Light Scattering Detector (ELSD) provides a wide detection range for a variety of compounds with excellent sensitivity, down to low-nanogram levels, better efficiency and greater reproducibility. It is perfect for the analysis of semivolatile and nonvolatile compounds and compounds without UV chromophores such as most amino acids and sugars. The 1290 Infini...
متن کاملPurity Assessment of Drug Discovery Compound Libraries Using an Agilent Single Quadrupole LC/MS System Coupled to Diode Array and Evaporative Light Scattering Detectors
Medicinal and analytical scientists supporting pharmaceutical medicinal chemistry efforts usually assess the purity of synthesized compounds prior to selecting them for biological activity. This application note demonstrates a fast and automated purity assessment of a 30 compound library using one generic method and a multidetector system. The use of the Agilent 6130 Series Single Quadrupole LC...
متن کاملDetection of fumonisins produced in Fusarium moniliforme cultures by HPLC with electrospray MS and evaporative light scattering detectors.
A variety of toxic secondary metabolites including fumonisins, can be produced by the fungus Fusarium moniliforme and closely related species in section Liseola in large amounts (g/kg in laboratory cultures). Underivatized fumonisins were detected by HPLC using either an evaporative light scattering detector or electrospray MS. Electrospray MS used together with NMR and GC/MS was used to identi...
متن کاملUniversal Quantification of Structurally Diverse Natural Products Using an Evaporative Light Scattering Detector
A lack of good methods for absolute quantification of natural products has limited the accuracy of high-throughput screening. Many currently used methods for quantification are either too slow or not amenable to the structural diversity of natural products. Recent developments in low-temperature evaporative light scattering detectors (ELSD-LT) have overcome several historical limitations of ELS...
متن کاملSimultaneous determination of bioactive constituents in Danggui Buxue Tang for quality control by HPLC coupled with a diode array detector, an evaporative light scattering detector and mass spectrometry.
Danggui Buxue Tang (DBT), a classical traditional Chinese formula comprising Radix Angelicae Sinensis (RAS) and Radix Astragali (RA), has been widely used to treat menopausal irregularity in Chinese women for nearly 800 years. In this study, a comprehensive analytical method of simultaneously determining the main types of bioactive constituents, eighteen in all from the formula, involving flavo...
متن کامل